课程-安徽省网络课程学习中心（e会学）

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基因工程是生物工程的核心技术，以微生物学、生物化学、分子生物学为基础，综合各种有关知识的应用技术学科。重点论述基因工程的基本原理及其单元操作，内容涉及 DNA 重组技术、分子克隆技术、外源基因的稳定高效表达技术等，以拓宽学生生命科学的知识面，为日后熟练驾驭这项技术服务于科学研究及国民经济打下坚实的基础。是实践性，应用性很强的专业课。

课程概述

课程大纲

Chapter 1. Introduction and Course Overview
- 1.1 The concept and birth of GE
- 1.2 The application of GE
- 1.3 Safety and Ethical Discussions
- 1.1The concept and birth of GE
- 1.2The application of GE
- 1.3 Safety and Ethical Discussions
- 第一章习题及答案
Chapter2: Enzymes used in genetic engineering
- 2.1.1 Restriction endonuclease
- 2.1.2 Typical Restriction Digest
- 2.1.3Restriction Maps
- 2.2DNA ligase and joining DNA molecules
- 2.3 DNA Polymerase and modifying enzymes
- 2.4 Nucleases
- 2.1.1 Restriction endonuclease
- 2.1.2 Typical Restriction Digest
- 2.1.3 Restriction Maps
- 2.2 DNA ligase and joining DNA molecules
- 2.3 DNA Polymerase and modifying enzymes
- 2.4 Nucleases
- 第二章习题及答案
Chapter3 Vectors
- 3.1 The characters and types of vectors
- 3.2.1 Plasmid biology and isolation
- 3.2.2 Popular plasmid vector
- 3.3Phage λ cloning vectors
- 3.4 Single-stranded phages vector
- 3.5 Vectors for cloning large fragments of DNA
- 3.1 The characters and types of vectors
- 3.2.1 Plasmid biology and isolation
- 3.2.2 Popular plasmid vector
- 3.3Phage λ cloning vectors
- 3.4 Single-stranded phages vector
- 3.5 Vectors for cloning large fragments of DNA
- 第三章习题及答案
Chapter4 Basic techniques
- 4.1.1Total cell DNA extraction
- 4.1.2 Measurement of DNA concentration and DNA Purification
- 4.1.3 the preparation of RNA
- 4.2.1 Agarose gels
- 4.2.2 Polyacrylamide Gel Electrophoresis01
- 4.3 PCR
- 4.4 Site-directed mutagenesis
- 4.5.1 Ways of Molecular Hybridization
- 4.5.2 Probe labeling
- 4.1.1Total cell DNA extraction
- 4.1.2 Measurement of DNA concentration and DNA Purification
- 4.1.3 the preparation of RNA
- 4.2.1 Agarose gels
- 4.2.2 Polyacrylamide Gel Electrophoresis
- 4.3 PCR
- 4.4 Site-directed mutagenesis
- 4.5.1 Ways of Molecular Hybridization
- 4.5.2 Probe labeling
- 第四章习题及答案
chapter 5 Gene of interest isolation and Cloning
- 第五章习题及答案
- 5.1.1 Isolation of Gene of Interest
- 5.1.2 Constructing a genomic and cDNA library
- 5.2.1 Sequence-dependent screening
- 5.2.2 Difference cloning
- 5.2.3 Screening expressing libraries
Chapter6:Introduction of DNA into living cells
- 6.1 Introduction of DNA into bacterial cells
- 6.2 Identification of recombinants
- 6.1Introduction of DNA into bacterial cells
- 6.2 Identification of recombinants
- 第六章习题及答案
chapter 7 Plant Genetic Engineering
- 第七章习题及答案
- 7.1 Overview of transgenetic plant
- 7.2.1Characteristics of Agrobacteria and Classes of Transformation Vectors
- 7.2.2Agrobacterium Protocols---Transformation
- 7.2.3Direct gene transfer methods
- 7.3Analyzing the Transgenic Plants
- 7.4Strategy in Improvement of Plant Properties
chapter8 Genetic Engineering of Animals
- 8.1 Transgenic animals
- 8.2 gene therapy
- 第八章习题及答案
chapter9 Achieving high-level expression of genes in host cell
- 9.1.1 Prokaryotic Expression Systems
- 9.1.2外源基因在真核细胞中的表达
- 9.2Strategies for Analyzing Gene Expression
- 9.3 Methods for Identifying biological functions of genes
- 第九章习题及答案
1-4章生物工程1班
1-4章生工2班
1-4章信息学院考试

授课目标

预备知识

配套教材

基因工程

郑振宇著

华中科技大学出版社

参考教材

Gene Cloning and DNA Analysis

Seventh Edition

T.A.BROWN 著

JohnWiley&Sons,Ltd

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授课教师

宋运贤

淮北师范大学